皮下注射肾上腺素复制气滞血瘀模型的血流动力学初探

Brief exploring of hemodynamics in model with Qi stagnation and blood stasis induced by subcutaneous injection of epinephrine

[目的]探讨皮下注射肾上腺素所致气滞血瘀模型的血流动力学变化。[方法]将24只大鼠分成肾上腺素组、生理盐水组、正常组,对各组大鼠连续2 h监测血流速度、心率、平均动脉压等血流动力学变化。[结果]皮下注射肾上腺素后,大鼠颈总动脉(CCA)血流速度下降(P<0.05),心率随时间无明显变化(P>0.05),CCA120 min平均动脉压较40 min降低(P<0.05)。肾上腺素组血流速度在60、80、100、120 min等时间点较正常组或生理盐水组均降低(P<0.05)。肾上腺素组心率较生理盐水组在60、80、100 min等时间点有所加快(P<0.05)。生理盐水组和肾上腺素组平均动脉压在0 min较正常组降低(P<0.05)。[结论]血流速度可作为肾上腺素所致气滞血瘀模型的量化评价指标,皮下注射肾上腺素后1 h是运用该模型进行活血化瘀药筛选与评价的较佳时间。

[Objective] To investigate the hemodynamic changes of Qi stagnation and blood stasis caused by adrenaline injected subcutaneously.[Methods] Twenty-four rats were randomly divided into epinephrine group,normal saline group,normal group.All the rats were continuously monitoring hemodynamic changes of blood flow velocity,heart rate,mean arterial blood pressure for 2 h.[Results] After subcutaneously injection of adrenaline,blood flow velocity of rat common carotid artery（CCA） decreased（P0.05）;heart rate was not significant different with time changing（P0.05）;CCA mean arterial pressure at 120 min was lower than at 40 min（P0.05）.The blood flow in epinephrine group at 60 min,80 min,100 min,120 min was lower than the normal group or the normal saline group（P0.05）.The heart rate in epinephrine group was higher than that of normal saline group at 60 min,80 min and 100 min（P0.05）.CCA mean arterial pressure at 0 min in normal saline group and epinephrine group were lower than normal group（P0.05）.[Conclusion] Blood flow velocity can be used as one of the quantitative evaluation indicators of Qi stagnation and blood stasis caused by adrenaline,One hour after adrenaline injection was a apposite time for screening and evaluating the drug effect of blood-activating and stasis-dissolving with this model.