银杏叶提取物EGB761对阿尔茨海默病家兔模型ACAT1和LCAT mRNA表达的影响

Effect of ginkgo biloba extract EGB761 on the mRNA expression of ACAT1 and LCAT in rabbit with Alzheimer's disease

[目的]观察银杏叶提取物EGB761对阿尔茨海默(AD)病家兔模型胆固醇酰基转移酶(ACAT1)和卵磷脂-胆固醇酰基转移酶(LCAT)mRNA表达的影响。[方法]以2%高胆固醇饲料喂养雄性新西兰大白兔,建立AD家兔模型。模型建立后,用50 mg/(kg.d)EGB761灌胃3周作为EGB761组,以5 mg/(kg.d)辛伐他汀作为阳性药对照组。干预结束后,通过实时定量逆转录-聚合酶链反应(RT-PCR)法检测海马卵磷脂-胆固醇酰基转移酶mRNA和胆固醇酰基转移酶1 mRNA的表达。[结果]银杏叶提取物EGB761可以下调ACAT1 mRNA的表达,不影响LCAT mRNA的表达。[结论]银杏叶提取物EGB761可下调AD家兔模型ACAT1 mRNA表达,对LCAT mRNA表达无调节作用。

[Objective] To observe whether Ginkgo biloba extract EGB761 can influence the expression of ACAT1 and LCAT mRNA in rabbit with Alzheimer＇s disease.[Methods] The male adult New Zealand rabbits were fed by 2% high cholesterol feed in order to establish the model of Alzheimer＇s disease.EGB761 was used to treat the rabbits at a dose of 50 mg/（kg·d） for 3 weeks as EGB761 group.Simvastatin was fed at a dose of 5 mg/（kg·d） as a positive control drug.After intervention,the content of lecithin-cholesterol acyl transferase mRNA and cholesterol acyltransferase mRNA in hippocampus was tested by means of real-time quantitative RT-PCR.[Results] The content of ACAT1 mRNA in hippocampus was reduced by Ginkgo biloba extract EGB761,P0.01 compared with model group.But the content of ACAT1 mRNA was not significant different compared with model group（P0.05）.[Conclusion] Ginkgo biloba extract EGB761 can down-regulate the expression of ACAT1 mRNA in rabbit with Alzheimer＇s disease,however,it has no effect on the expression of LCAT mRNA.