摘要
为了找到小鼠卵巢颗粒细胞更加合适的体外生长环境,本研究分别向分离的小鼠卵巢颗粒细胞中加入无血清RM-PI1640、含10%血清RMPI1640、无血清DMEM(高糖)、含10%血清DMEM(高糖)及含15%血清DMEM(高糖)的培养液对小鼠卵巢颗粒细胞进行体外培养。试验经观察结果显示,DMEM(高糖)总体培养效果好于RMPI1640,且含15%血清的DMEM(高糖)是较理想的培养用液。
The purpose of granulosa cells in mice to find a suitable environment for the growth in vitro.Respectively,separated by experimental mouse ovarian granulosa cells after serum RMPI1640,with 10% serum RMPI1640,serum DMEM(high glucose),with 10% serum DMEM(high glucose) and containing 15% serum DMEM(high glucose) cultured in this study.The results showed that DMEM(high glucose) culture was generally better than RMPI1640,and containing 15% serum DMEM(high glucose) was an ideal medium.
出处
《中国畜牧兽医》
CAS
北大核心
2011年第2期107-110,共4页
China Animal Husbandry & Veterinary Medicine
基金
国家自然基金项目资助(30871792)