猪伪狂犬病病毒gE基因缺失苗免疫试验

The Immunity Test of gE Gene Deleted Vaccine of Pseudorabies Virus

为了掌握猪伪狂犬病gE基因缺失疫苗免疫效果,并制定合理的免疫程序,选用国产和进口的猪伪狂犬病gE基因缺失弱毒疫苗,用4种不同的免疫程序,对250头母猪和1000头仔猪进行了免疫试验。试验期间,按比例定时采集免疫猪的血液,用ELISA试剂盒进行抗体检测,证明猪伪狂犬gE基因缺失疫苗,无论国产苗或进口苗都可以产生良好的免疫效果;无论跟胎免疫、1年2次普免,每隔4个月定时免疫,效果均良好。种猪免疫抗体合格率达100%,仔猪49日龄前抗体合格率达100%,75日龄后抗体逐渐降低,120日龄后基本消失。为了使猪体免疫力更强,不给野毒入侵的机会,建议仔猪在首免后,适当时间进行二次加强免疫。而只给公猪、母猪春秋两季免疫,不给仔猪免疫组,仔猪在35日龄后抗体降为阴性,不能抵抗野毒的侵袭,这种免疫方法不宜推广。

In order to master the immune effectiveness of gE gene deleted vaccine of pseudorabies virus and formulate rational immunization programs,the immunity tests had been done with domestic and imported gE gene deleted attenuated vaccine of pseudorabies virus by 4 different immune procedures in 250 sows and 1000 piglets.During the trial,according to the proportion of regular collecting the blood of immune pigs,the antibodies were detected by ELISA diagnostic kits.Trials had proved that whether domestic or imported gene deleted vaccine of pseudorabies virus could produce a good immune effects which were good whether immunization with the child,or twice a year,or every 4 months.Boar antibodies passed rate of 100%,the piglets antibodies passed rate of 100% before 49-day-old,decreased gradually after 75-day-old and disappeared after 120-day-old.To make a stronger immune system of pigs and don＇t give the opportunity of the wild poisonous invasion,it proposes the appropriate time for the second booster immunization after the first immunization of the piglets.Only the boars and sows were immunized in spring and autumn,but the piglet group was not immunized and the antibodies of piglets will reduce negative after 35 days,which can not resist the wild poisonous invasion,this method of immunization should not be promoted.