野生东北刺人参茎挥发油成分及其抗皮肤癣菌作用

Chemical constituents of essential oil in stem of Oplopanax elatus and their antifungal action

目的：鉴定野生东北刺人参茎挥发油成分并观察其抗皮肤癣菌活性。方法：按中国药典１９９５年版规定的方法提取挥发油，用ＧＣＭＳ和ＧＣＩＲ鉴定化学成分，采用试管内药基法进行抗皮肤癣菌试验。结果：从野生东北刺人参茎中提得３１％的挥发油，鉴定了其中３２种化合物，有２５种为首次从该植物中鉴定。主要成分为α蒎烯（６２％），辛醛（８７％），６，６二甲基２２亚甲基原蒎烷（６１％）和５甲基己醛（５９％）。野生东北刺人参茎挥发油对红色毛癣菌等７种皮肤癣菌抗菌活性ＭＩＣ为００６３％～０１２５％，ＭＦＣ为０１２５％～０２５％。抗皮肤癣菌有效成分有里哪醇（２４％）和对聚伞花素（１８％）等。结论：野生东北刺人参茎挥发油含量较高，含有多种抗皮肤癣菌有效成分，其抑杀皮肤癣菌活性显著，可作为开发抗皮肤癣菌新药的研究依据。

OBJECTIVE: To identify the chemical composition of essential oil in the stem of Oplopanax elatus and observe its antifungal activity METHOD: The stem was steamdistilled according to the China Pharmacopoeia to extract the oil The chemical constituents were identified by GCMS and GCIR Antifungal studies were carried out in vitro RESULTS: The stem yielded 31% of the oil 32 compounds were identified, 25 compounds of them were identified first from oplopanax elatus The major components of the oil were pinene (62%); octanal (87%);6,6Dimethyl2methylenenorpinane (61%), and 5Methyl hexanal (59%) MIC of the oil against 7 pathogenic dermatophytes ranged from 0063%-0125% and MFC 0125%-025% The known effective constituents are Linalol and Pcymene from the oil against pathogenic dermatophytes CONCLUSION: The oplopanax elatus contains high coutent of essential oil Most ofthe compositions in the oil are Aldehydes and Cyclic Terpenes The oil showed a significant antifungal activity aganist Trichophyton rubrum, T v