摘要
为了给医学实验用猪的选育工作提供相关资料，作者采用聚合酶链反应结合限制性内切酶（ＰＣＲ－ＲＦＬＰ）技术对２５头中国内江猪的氟烷基因型进行了检测，结果２５头内江猪氟烷基因型均为阴性纯合子。从而提示中国内江猪有可能成为医学实验动物及猪→人异种移植的选育猪种之一；ＰＣＲ－ＲＦＬＰ法是鉴别猪氟烷基因型的可靠又简便的方法。
The aim of this study was to test the RYR1 genotype of 25 Neijiang pigs ao as to provide data for the selective breeding of medical experimental pig strains. PCRRFLP technique was used. The results showed that the RYR1 genotype of all the 25 porcine cases was HALNN and all of the 25 porcine cases were Halothane Negative. These suggested that Neijiang pig could be one of the procine strains selectively bred for medical experiments and clinically for pigtohuman xenotransplantation. PCRRFLP technique is a reliable and simple method for RYR1 gene testing.
出处
《华西医科大学学报》
CSCD
1999年第2期117-119,共3页
Journal of West China University of Medical Sciences
基金
国家自然科学基金
四川省重大科技攻关课题
