摘要
对已建立的八肽胆囊收缩素(CCK8)酶联免疫吸附试验的应用作初步探索。结果显示:相关肽2Ag、3Ag及CCK8之抑制曲线斜率有异,分别为-0.46、-0.17、-0.68;脑组织提取物可被CCK8定量抑制;CCK8不与其他4种八肽抗血清发生反应,表明该法可用于对CCK相关肽进行初步结构分析,亦可用于组织提取物CCK成分鉴定及多肽抗血清筛选,因而具有较好的应用前景。
An enzymelinked immunosorbent assay for octapeptide cholecystokinin (CCK) has been developed. Using this method we distinguished two kinds of CCKrelated peptides and is also useful for identified the compounds with CCKlike structure in tissue extracts, as well as different antipeptide antisera. The results showed that this method could be applied to preliminary structure analysis of CCK.
出处
《华西医科大学学报》
CAS
CSCD
1999年第2期217-219,共3页
Journal of West China University of Medical Sciences