慢性乙型肝炎患者血清学指标综合评价

The Total Estimation of Serum Indicator in Patients with Chronic Viral Heptatitis Type B

目的全面检测慢性乙型肝炎(乙肝)患者各项血清学指标,以期筛选出更经济、实用的随访指标。方法用酶联免疫吸附试验检测260例慢性乙肝患者血清病毒标志物(HBV-M)、前S1抗原(PreS1-Ag),用荧光定量-聚合酶联反应方法检测HBV DNA含量,用全自动生化分析仪检测肝功能指标。结果慢性乙肝患者血清PreS1-Ag与HBV DNA拷贝数具有良好的正相关性;HBeAg阴性组PreS1-Ag阳性率为54.7%,HBV DNA阳性率为56.0%;慢性乙肝患者肝功能指标中的ALT、AST、TP、A/G、GGT阳性率较其他指标高,其中ALT阳性率最高(58%),AST次之(42%)。结论 PreS1-Ag与HBV DNA符合率较高,比HBeAg更能反应病毒复制情况,ALT、AST是较灵敏的肝功能指标。慢性乙肝患者可以PreS1-Ag、ALT及AST作为长期随访指标。

Objective In order to select economical and valuable follow-up indicator,all serum indicator of patients with chronic HBV were detected. Methods The HBV markers,PreS1-Ag were detected by ELISA in 260 patients with HBV. The level of HBV DNA were detected by fluorescence quantitative PCR. The level of liver function were obtained by an automated biochemistry analy- zer. Results No significant difference was found between the detectable rates of HBV DNA and PreS1 Ag in patients with chronic heptatitistry virus The positive rate of PreS1-Ag and HBV DNA were 54.7% and 56.0% in HBeAg-nagative group. Significant difference was found between the detectable rates of ALT, AST, TP, A/G, GGT in patient with HBV and the detectable rates of ALB,G,TBIL,DBIL,ALP. The positive rate of ALT and AST were 58% and 42.% respectively. Conclusion HBV PreS1-Ag closely correlates with HBV DNA. ALT and AST are the most sensitive liver function markers, the patients with chronic heptatitis B virus can select PreS1-Ag,ALT and AST as the long follow-up indicator.