摘要
目的用两种免疫学方法检测乙肝患者血清中的乙肝五项,探讨HBVs系统(HBsAg/HBsAb)和HBVe系统(HBeAg/HBeAb)同时阳性的情况。方法 568例乙肝患者血清分别用酶联免疫吸附试验(ELISA)和电化学发光免疫技术(ECLIA)进行检测。结果 HBVs系统双阳性检出率ELISA和ECLIA分别为3.69%和5.81%,14例HBsAg或HBsAb浓度呈较低水平的标本,ELISA检测为阴性,ECLIA检测则为阳性,两种方法检测结果间差异有统计学意义;HBVe系统双阳性检出率ELISA和ECLIA分别为7.57%和11.44%,25例HBeAg或HBeAb浓度呈较低水平的标本,ELISA方法检测为阴性,ECLIA法检测则为阳性,两种方法检测结果间差异有统计学意义。结论 ECLIA在HBVs和HBVe系统检测中比ELISA具有更高的灵敏度,可以更灵敏地识别早期HBV亚型的感染或HBsAg向HBsAb的早期转变;而对于HBVe系统的血清转换,ECLIA比ELISA能更好地发挥病程监测和疗效观察的作用。
Objective Using two immunological methods in the detection of hepatitis B patients with serum hepatitis B in order to explore HBVs System(HBsAg/HBsAb)and HBVe System(HBeAg/HBeAb) positive cases at the same time. Methods 568 cases of hepatitis B patients with serum enzyme-linked immunosorbent assay(ELISA) were used and electrochemical luminescence immunoassay(ECLIA) for testing. Results HBVs system in double-positive detection rate of antigen-antibody ELISA method and ECLIA method were 3.69% and 5.81%, 14 cases of HBsAg or HBsAb concentration content to a lower level of specimens, EIASA testing methods {or the negative, and the detection of ECLIA positive,two methods by statistical significant difference(P^0.01) ; HBVe system in double-positive detection rate of antigen-antibody ELISA method and ECLIA are 7.57~ ,and 11.44~ respective- iy,25 cases of HBeAg or the concentration of HBeAb content of the samples showed lower levels, ELISA detectresult negative, while the ECLIA assay was positive,two methods by statistical significant difference(P〈0.01). Conclusion ECLIA method HBVs and HBVe system antigen-antibody detection ELISA,can be more sensitive to identify early HBV type infections or HBsAg to HB sAb early change;For HBVe system seroconversion,ECLIA is better fhan the ELISA method and can play a course of monitoring and efficacy of the clinical application.
出处
《国际检验医学杂志》
CAS
2011年第2期179-180,共2页
International Journal of Laboratory Medicine