苦参碱对纤维蛋白纤维蛋白原降解产物诱导血管细胞损伤、增殖及腹腔巨噬细胞释放IL-1的影响

EFFECTS OF MATRINE ON THE INJURY AND PROLIFERATION OFVASCULAR CELLS AND RELEASE OF INTERLEUKIN1 FROMPERITONEAL MACROPHAGES CAUSED BY FIBRIN FIBRINOGEN DEGRADATION PRODUCTS

目的：研究苦参碱（Ｍａｔ）对纤维蛋白纤维蛋白原降解产物（ＦＦＤＰ）作用的影响。方法：大鼠主动脉内皮细胞损伤以乳酸脱氢酶释放测定；大鼠主动脉平滑肌细胞增殖采用结晶紫染色法测定；白细胞介素１活性采用小鼠胸腺细胞增殖法测定。结果：ＦＦＤＰ能促进大鼠主动脉内皮细胞释放乳酸脱氢酶，诱导大鼠主动脉平滑肌细胞增殖，并促使小鼠腹腔巨噬细胞分泌ＩＬ１增加。结论：Ｍａｔ可抑制ＦＦＤＰ的作用。对动脉粥样硬化的防治可能有一定意义。

AIM: To study the effects of matrine on rat aortic endothelial cell injury, rat aortic smooth cell proliferation and release of interleukin1 from mouse peritoneal macrophages induced by fibrin fibrinogen degradation products(FFDP). METHODS: Rat aortic endothelial cell injury was assayed by lactic dehydrogenase release. Rat aortic smooth muscle cell proliferation was determined by crystal violet staining assay. Interleukin1 activity was measured by mouse thymocyte proliferation assay. RESULTS: Fibrin fibrinogen degradation products significantly increased the lactic dehydrogenase release from rat aortic endothelial cells, proliferation of rat aortic smooth muscle cells and release of interleukin1 from mouse peritoneal macrophages. Matrine exerted inhibitory effects on the action of fibrin fibrinogen degradation products. CONCLUSION: Fibrin fibrinogen degradation products may play an important role in atherosclerosis plaque formation, and matrine has protective effects against the atheroscler