苯丙氨酸对自发性高血压大鼠血管平滑肌细胞内钙的影响

L-phenylalanine effects on intracellular calcium in vascular smooth muscle cell of spontaneously hypertensive rat

目的：探索苯丙氨酸对自发性高血压大鼠血管平滑肌细胞内钙的影响。方法：将ＳＨＲ和ＷＫＹ的血管平滑肌细胞在不同浓度苯丙氨酸培养液中培养一定时间后，加入［４５ＣａＣｌ２］温育一定时间后，测定进入细胞内［４５Ｃａ２＋］的量；而后将在不同浓度苯丙氨酸培养液中培养一定时间后的ＳＨＲ和ＷＫＹ的血管平滑肌细胞同ＰＳＳ液温育，采用Ｆｕｒａ－２／ＡＭ荧光指示剂测定不同浓度苯丙氨酸对细胞内游离Ｃａ２＋的影响。结果：苯丙氨酸浓度越高，进入ＳＨＲ血管平滑肌细胞内的钙和细胞内游离钙含量越少，但对ＷＫＹ无影响。结论：苯丙氨酸可以特异性地抑制Ｃａ２＋进入ＳＨＲ血管平滑肌细胞和使细胞内游离Ｃａ２＋减少。

AIM:To find the L-Phenylalanine (Phe) effects on intracellular calcium in vascular smooth muscle cell(VSMC) of spontaneously hypertensive rat (SHR) and WKY.METHODS:VSMC of SHR and WKY were incubated with phenylalanine in 7 different concentration for a period, and then added [ 45 CaCl 2] into each well and incubated for another 30 minutes. The VSMC in each well were washed and counts·min -1 of [ 45 CaCl 2] was measured. Similar to above, incubated VSMC cultured in different concentration Phe with PSS solution, and measured the intracellular free calcium concentration with Fura-2/AM. RESULTS:The higher L-phenylalanine concentration, the lesser calcium influx from the extracellular and intracellular free calcium concentration in VSMC of SHR. However, there were no variations in VSMC of WKY. CONCLUSION:Phenylalanine effect that reduces total intracellular free calcium concentration may contribute to its action in preventing blood pressure rise and the hypertrophy of vessel in SHR.