支气管哮喘发病中β_2AR mRNA水平变化的初步探讨

Study on the changes of β 2AR mRNA in bronchial asthma

目的：初步探讨β２ＡＲｍＲＮＡ在支气管哮喘发病中的变化。方法：用ＲＴ－ＰＣＲ及原位杂交方法测定哮喘时人外周淋巴细胞及大鼠肺组织β２ＡＲｍＲＮＡ水平。结果：缓解期哮喘患者β２ＡＲｍＲＮＡ水平与正常人无显著差异。发作期哮喘大鼠肺组织β２ＡＲｍＲＮＡ水平显著降低，且肺内不同组织细胞β２ＡＲｍＲＮＡ水平的下降程度并不一致。结论：提示哮喘时β２ＡＲｍＲＮＡ的变化可能是β２ＡＲ变化的分子基础，这对哮喘治疗具有重要的实际意义。

AIM:To preliminaryly inquire into the changes of β 2AR mRNA levels in bronchial asthma.METHODS: To measure the β 2AR mRNA levels in asthmatic peripheral lymphocytes and the lungs of challenged rats by using RT-PCR and in situ hybridization.RESULTS:(1) Expression of β 2AR mRNA in peripheral lymphocytes of asthmatics at remission stage was no significant differences compared with that in normal volunteers; (2) Expression of β 2AR mRNA in the lungs of challenged rats decreased markedly, but β 2AR mRNA levels among various tissues inside lung decreased to different extent after allergen challenge.CONCLUSION: Changes of β 2AR mRNA might underlie the changes of β 2AR in the lungs of asthmatics and challenged rats, and it also furnished theoretical basis for the useage of β 2AR agonists clinically.