1株烟碱降解菌的筛选、鉴定及其降解性能的初步研究

Isolation and characterization of a high nicotine-degradation bacterium

以烟碱为唯一碳源,从湖北省襄樊烟草种植地中分离得到1株烟碱降解菌,命名为DBA5。经常规的形态观察、生理生化分析和16S rDNA序列同源性分析,初步鉴定该菌株为烟碱节杆菌属(Arthrobacter nicotianae)。当烟碱质量浓度为4.0 g/L时,培养48 h烟碱降解率为93.32%,生长旺盛。当烟碱质量浓度为5.0g/L时,培养48 h烟碱降解率为65.10%。当烟碱质量浓度高于6.0 g/L时,DBA5菌株生长和烟碱降解均呈下降趋势,烟碱降解率低于7.56%。

Thirty bacterial strains were isolated from soil using nicotine as the only carbon source and a high nicotine degradation bacterium strain,named as DBA5,was screened out from them.Based on standard morphologica1,physiological properties and 16S rDNA analysis,DBA5 was identified as Arthrobacter nicotianae.When the medium with 4.0 g/L nicotine,DBA5 could grow well and degrade 93.32% of nicotine in 48 h.When the medium with 5.0 g/L nicotine,it degrade 65.10% of nicotine in 48 h.The growth and nicotine degradation of DBA5 was declined when nicotine concentration was more than 6.0 g/L,and it degraded less than 7.56% of nicotine.DBA5 showed high ability to degrade nicotine.