加拿大进境油菜籽茎基溃疡病菌的检测与鉴定

Detection and identification of Leptosphaeria maculans in imported Canadian rapeseeds

从加拿大进境油菜籽中随机选取船载油菜籽样品,通过特异引物的PCR扩增检测和病原菌分离鉴定,观察了油菜茎基溃疡病菌Leptosphaeria maculans的发生情况。结果表明:所有来自3艘轮船的15份油菜籽样品的油菜茎基溃疡病菌PCR检测都呈阳性;随机选择的3份阳性样品的病原菌分离、鉴定与致病性测试结果证实了PCR的检测结果。

In this research, shipborne samples were randomly selected from imported Canadian rape- seeds to investigate the occurrence of phoma stem canker（Leptosphaeria maculans） by PCR amplifica- tion,pathogen isolation and pathogenicity test. The results showed that all fifteen rapeseed samples from three ships were positive in PCR detection of Leptosphaeria maculans. The results from pathogen isolation, identification and pathogenicity testing of three randomly selected samples confirmed the PCR detection.