不同靶点shRNA干扰对虾白斑综合征病毒增殖效果分析

Effect of the viral interference effects of shRNAs targeting different sites in white spot syndrome virus genome

设计合成了靶向对虾白斑综合征病毒(WSSV)基因组不同位点的5个shRNA:RR9、RR1、DP1、DP2和PK1,并在凡纳滨对虾体内实施了RNA干扰(RNAi)抗WSSV增殖的试验。结果显示:5个shRNA不同程度地抑制了WSSV的复制,降低了对虾的死亡率,其中,靶向病毒核糖核酸还原酶的shRNA RR9的RNA干扰效果最佳。

Five shRNAs targeting different sites in white spot syndrome virus （WSSV） genome,respeetively designated as RR9, RR1, DP1, DP2 and PK1, were designed and constructed. Followed by WSSV infection,each of which was introduced into Litopenaeus vannamei shrimp by injection. The result demonstrated that both the proliferation of WSSV in vivo and the shrimp mortality were decreased in different degrees in all of the shRNAs-treated groups. Based on the analysis, the RR9 targeting WSSV ribonueleotide reductase gene had the best protective effect against WSSV, which would be used for a further research on anti-WSSV by RNAi.