响应面法优化白骨壤果实中黄酮类化合物的提取工艺

Optimization of Extraction Technology of Flavonoids from Avicennia marina Fruits by Response Surface Methodology

本文研究利用响应曲面法优化白骨壤果实中黄酮类化合物的提取工艺。在单因素试验的基础上,利用中心组合设计响应面试验,研究了提取温度、提取时间、乙醇浓度和料液比(m/V)对白骨壤果实黄酮类化合物提取率的影响,并建立了回归模型。结果表明,最佳提取工艺条件为提取温度54.4℃、提取时间3.45 h、乙醇体积分数65.68%、料液比1:21.81(m/V),该工艺条件下白骨壤果实中黄酮类化合物的理论提取率为8.651%,实测提取率为8.613%,回归模型预测与实际情况吻合良好。

Response surface methodology （RSM） was used to optimize the extraction technology of flavonoids from Avicennia Marina fruits in this paper. On the basis of single factor tests, a central composite design （CCD） experiment was conducted and the response surface analysis was employed to investigate the influence of four main factors including temperature, extracting time, concentration of ethanol, ratio of solvent to raw material on the yield of flavonoids, and then a regression model established. The results showed that the optimum extraction conditions were temperature 54.4 ℃, time3.45 h, concentration of ethanol 65.68%（v/v） and ratio of solvent to raw material 21.81 mL/g. The predicted yield of flavonoids with the optimized procedure was 8.651%, while the practical yield of flavonoids was 8.613%, indicating a good fitness of the regression model and the actual process.