油茶种子脂肪酸与游离氨基酸的分析

Fatty acid and amino acid composition of Camellia oleifera seeds

以4个油茶良种种籽为试材,采用索氏法提取油茶种子中的油脂,运用气相色谱分析测定油茶种子中的脂肪酸的相对含量,利用氨基酸自动分析仪测定其氨基酸的组成及含量。结果表明：不同良种间脂肪酸的相对含量差异显著,‘华硕、华金、华鑫、湘林XLC15’其不饱和脂肪酸相对含量高达89.15%;棕榈酸相对含量介于9.39%~13.18%;硬脂酸介于1.46%~2.86%。4个油茶种子中均含有17种游离氨基酸,其中总氨基酸含量最高者为‘华硕’是673.1 mg.100 g-1,次之为‘华鑫’是583.9 mg.100 g-1,最低为‘华金’是404.6 mg.100 g-1。不同良种间在精氨酸的含量差异显著,其他氨基酸差异不显著。

By take four Camellia oleifera superior clones as the testing materials,Camellia oleifera seeds oil was extracted with Soxhlet extraction,fatty acid composition of the oil was analyzed with gas chromatography,and the amion composition was analyzed with automatic andyzer.The results show that the relative content difference of fatty acids between clones was quite obvious,the relative content of unsaturated fatty acids was as high as 89.15%;the relative content of palmitic acid ranged from 9.39%to 13.18%;hard fatty acid among 1.46%and 2.86%.Four Camellia seeds contained 17 kinds of free amino acids,of which the highest total amino acid content ＇Huashuo＇ was 673.1 mg·100 g-1,followed by the ＇Huaxin＇to be 583.9 mg·100 g-1,the lowest for the＇Huajin＇ 404.6 mg·100 g-1.The differences in arginine content among the four fine varities were notable,and the differences in other amino acids content were not abvious.