高压液相色谱法测定食品中的黄曲霉毒素B_l

Determination of Aflatoxin B1 in Food with HPLC

本文应用ＨＰＬＣ－ＦＬＤ，在激发波长３６５ｎｍ、荧光波长４５０ｎｍ，流动相为甲醇／水１∶１、流速１．０ｍｌ／ｍｉｎ，色谱柱为Ｈｙｐｅｒｓｕｌ，ＯＤＳ５μｍ、１２５×４ｍｍ条件下，测定黄曲霉毒素Ｂ１衍生物－黄曲霉毒素Ｂ２ａ［１］，得到ＡＦＴＢ１浓度与峰面积成正比的工作曲线（相关系数ｒ＝１．０００）。本法精密度、准确度好，灵敏度高，其变异系数ＣＶ为２．５％，回收率范围为９５％～１０１％，检出限为０．２ｎｇ。

In the present study,HPLCFLD was used to examine the aflatoxin B1 derivativeB2ain food,with excitation and fluorescence wave-lengths 365nm and 450nm respectively,methanol/H2O=11 in the mobile phase and a flow rate 10ml/min,1254mm Hypersil column packed with ODS5um. A working curve showing a direct ratio (r=1.000) of the concentration against the peak area of AFTB1 was obtained.The method proved to be satisfactory in precision,accuracy and sensitivity,with CV=25%,recovery rate 95%￣101% and detection limit 02ng.