双波长薄层扫描法测定湖北贝母中湖贝甲素的含量

Determination of Content of Hupehenine in Hubei Beimu by TLC-Scanning

湖北贝母为百合科植物Fritillaria hupehensis Hsiao et K.C.Hsia的干燥鳞茎,拟收载于《中国药典》2000年版,为了控制药材质量,本文采用双波长薄层扫描法测定了湖北贝母中湖贝甲素(Hupehenine)的含量,用冷浸法提取,以苯-乙醚-醋酸乙酯-二乙胺(3:3:4:1)为展开剂,喷以稀碘化铋钾试液,双波长反射法锯齿扫描,λ_S=500nm,λ_R=600nm,回收率为101.4％,RSD=4.4％,经测定9批样品,含量为0.028％～0.187％,表明不同产地样品含量差异较大,建议暂订湖北贝母中湖贝甲素含量应不低于0.03％。

The content of hupehenine in Fritillaria hupehensis Hsiao et K. C. Hsia was determined by TLC-scanning. The samples were extracted with ether by cold maceration method. The extract was developed with banzene-ether-ethyl acetate-diethy-lamine(3:3:4:1) and sprayed with dilute postassium iodobismuthate TS. The plate was scanned at 500 nm(λs) and 600 nm(λR) by refletion-zigzag mode. The average recovery was 101.4 % (from 0.028 % to 0. 187 % , n =9), RSD = 4.4%.