川续断对Alzheimer病模型大鼠海马内淀粉样前体蛋白表达的影响

EFFECT OF DIPSACUS ASPER ON THE EXPRESSION OF AMYLOID PRECURSOR PROTEIN IN THE DORSAL HIPPOCAMPAL FORMATION IN RAT MODEL OF ALZHEIMER DISEASE

有关Ａｌｚｈｅｉｍｅｒ病（ＡＤ）的治疗尚无有效的措施。文中用铝诱导建立ＡＤ大鼠模型，将ＡＤ大鼠分为对照、续断和ｖｉｔａｍｉｎＥ（ＶＥ）三组，用川续断／ＶＥ处理ＡＤ大鼠１，３，５个月，分别进行行为学测试、光镜形态学观察、免疫细胞化学图像分析处理。结果：１．川续断／ＶＥ处理１，３，５个月三个时间段中三组间①大鼠学习记忆力有显著性差异（Ｐ＜０．０５）；②海马结构内淀粉样前体蛋白样免疫反应（ＡＰＰ－ＬＩ）神经元的胞体平均截面积及光密度（ＡＳＯ）各存在有统计学差异（Ｐ＜０．０５）。２．续断组和ＶＥ组于川续断／ＶＥ处理１，３，５个月间的纵向比较显示①大鼠学习记忆力也有显著性差异（Ｐ＜０．０５或０．０１）；②海马结构内ＡＰＰ－ＬＩ神经元的胞体平均截面积及ＡＳＯ各存在有统计学差异（Ｐ＜０．０１）。提示川续断和ＶＥ对淀粉样前体蛋白在神经元的过度表达有明显的抑制作用，并且可以改善大鼠学习记忆力。

There is not an effective cure for Alzheimer disease (AD). In this paper,the model of dementia was established by pretreatment with aluminium chloride,the AD rats were divided into control,dipsacus asper,vitamin E(VE) three groups and were dealt with dipsacus asper or VE for one,three,five months,behaviour test and light microscope morphological observation as well as immunocytochemical method combinating with image analysis were adopted.The results showed that:1.Among the three groups in three time periods which were dealt with dipsacus asper or VE for one,three,five months.①The learning and memorizing abilities of rat significantly different among three groups( P <0 05);②The average area and absorbance (ASO) of the amyloid precursor protein like immunoreactivity (APP LI) neurons in dosal hippocampal formation were significantly different among three groups( P <0 05).2.To make longitudinal comparison among groups which were dealt with dipsacus asper or VE for one,three,five months:①The learning and memorizing abilities of rat were also significant differences among three groups( P <0 05 or 0.01);②The average area and ASO of the APP LI neurons in dosal hippocampal formation were significant differences among three groups( P <0 01).These findings indicate that both dipsacus asper and VE can prevent the overexpression of the APP in dosal hippocampal formation,and improve the learning and memorizing abilities of rat.