背根神经节神经元胞体膜可能存在NMDA自身受体(英文)

POSSIBLE EXISTENCE OF NMDA AUTORECEPTOR IN CELL BODY MEMBRANE OF DRG NEURONS 1

应用经酶和机械消化分离的大鼠背根神经节（ＤＲＧ）神经元进行实验。首先运用全细胞膜片箝技术找到ＮＭＤＡ可引起一内向电流的神经元，此内向电流可被甘氨酸明显增强，并被ＮＭＤＡ受体的特异性拮抗剂ＡＰＶ完全阻断。随后，把经电生理技术证实膜上存在ＮＭＤＡ受体的这一ＤＲＧ神经元用微吸管转移到载玻片上，进行谷氨酸神经递质免疫组织化学检测。１０／１８的ＤＲＧ细胞呈免疫反应阳性，结果为ＤＲＧ神经元膜上存在ＮＭＤＡ自身受体提供了新证据。

In the present study we developed a practicable method by which the electrophysiological evidence for NMDA receptor in the cell body membrane was combined with immunocytochemical revelation of glutamate like immunoreactivity (Glu LI) in the same dorsal root ganglion(DRG) neuron.In brief,the neurons were isolated freshly from rat DRG by means of enzymatic (trypsin,collagenase,DNase)and mechanical treatment and the NMDA activated current was recorded by whole cell patch clamp technique.The current was potentiated markedly by coapplication of NMDA with glycine and blocked obviously by APV.Thereafter,the neuron in which the NMDA receptor was identified electrophysiologically was sucked into a micropipette and transferred onto a slide,on which the immunocytochemical procedures were done.With this method we have identified NMDA receptors in the membrane of some DRG neurons as autoreceptors.