摘要
对一株从土壤中筛选出来的产蓝色素的放线菌进行了初步鉴定, 命名为天蓝色链霉菌-100(Streptom yces coelicolor-100 ),并对蓝色素的性质进行了研究.结果表明此色素无臭无味,水溶性较强.酸性条件下,溶解度随pH 降低而减小.pH< 8 时为红色,pH≥8 时为蓝色,颜色随pH 增大而加深.于100 ℃短时间处理,比较稳定.在pH 中性的条件下对光(包括紫外光)较稳定.大多数金属离子如Ba2+ 、K+ 等对此色素无影响,Mg2+ 使色素增色.Fe2+ 在5×10-4 m ol/L时,使色素变性,但在5×10-5 m ol/L时,对色素没有多大影响.Pb2+ 可以络合色素,使之沉淀;加入0.25% 盐酸乙醇后,色素再溶解.该蓝色素可作为天然色素或天然pH 指示剂开发.
A blue-pigment producing Streptomyces isolated from soil was identified,the properties of the blue pigment were studied.The results showed that the blue pigment has no odor, and is well dissolved in water or methanol.The color of the pigment is red below pH 8 and blue above pH 8. It is relatively stable at 100 ℃ for a short time and under the lights including ultraviolet rays when the value of pH around 7. Metal ions such as Ba 2+ 、K + don't have any effect on the pigment stability. The concent ration of Fe 2+ make the pigment denatured above 5×10 -4 mol/l, a little effect below 5×10 -5 mol/l. Pb 2+ precipitate the pigment ,but the pigment redissolve after HCl-Ethanol was added.The pigment can be exploited as natural pigment or potential pH indicator.