微波辅助萃取-固相微萃取-气相色谱-质谱法分析花椒香气成分

Analysis of aroma components from Zanthoxylum bungeanum Maxin by microwave-assisted extraction coupled to headspace solid-phase microextraction and gas chromatography-mass spectrometry

采用微波辅助萃取与顶空-固相微萃取联合(MAE-HS-SPME)的方法萃取花椒香气成分,用气相色谱-质谱(GC-MS)法分析,并将分析结果与同时蒸馏萃取(SDE)和HS-SPME法对比。结果表明,MAE-HS-SPME的最佳条件为:称取10g样品,加入100mL去离子水,微波功率为120W,用100μmPA萃取头萃取5min,250℃解吸20s。GC-MS分析确认了59种成分,占总峰面积的96.65%;三种方法所得主要成分相同,均为芳樟醇、乙酸芳樟酯等;MAE-HS-SPME法鉴定出的成分较多,说明MAE-HS-SPME是一种高效的样品前处理方法。

The pretreatment technique of microwave-assisted extraction coupled to headspace solidphase microextraction （MAE-HS-SPME） was investigated on the extraction of aroma components from Zanthoxylum bungeanum Maxin. The extracted volatiles were analyzed by gas chromatographymass spectrometry（GC-MS） method, and the determination results were compared with that of simultaneous distillation and extraction （SDE） and HS-SPME methods. The results showed that the optimal extraction conditions of MAE-HS-SPME were extracting 5 min at 120 W microwave power from 10 g sample in 100 mL deionized water using a 100μm polyacrylate （PA） fiber, and then desorbing for 20 s at 250 ℃. The GC-MS analysis results showed that 59 components were identified and accounted for 96. 65% of the total peak areas. The main volatiles were the same as that separated from HS- SPME fiber and SDE method. The main volatiles were linalool, linalool acetate, etc. The total number of the components extracted by MAE-HS-SPME was more than that extracted by HS-SPME and SDE. The results inclieated that MAE-HS-SPME is a high performance pretreatment method.