蝎素组分Ⅲ对THP1细胞NF-κB转录因子的影响

Effects of SVC-Ⅲ on the expression of NF-κB in THP1 cells

目的:研究蝎素组分Ⅲ(Scorpion venom crudeⅢ,SVC-Ⅲ)对THP1细胞中NFκ-B转录因子的影响。方法:用RT-PCR方法检测THP1细胞中p65和IKK1基因的表达;Western blot检测细胞p65蛋白的表达;荧光素酶报告基因的方法观察NF-κB的转录活性。结果:1μg/L浓度的SVC-Ⅲ在mRNA和蛋白水平均对NFκ-B有促进表达作用,并增强NFκ-B的转录活性;10μg/L浓度的SVC-Ⅲ则未显示出促进作用;100μg/L浓度的SVC-Ⅲ反而表现出一定的抑制作用。结论:SVC-Ⅲ能以剂量依赖的方式调节THP1细胞NFκ-B转录因子的表达和活性。

Objective：To investigate the effect of SVC-Ⅲ on the expression of NF-κB in THP1 cells.Methods：The expression of p65 and IKK1 mRNA in THP1 cell was measured with RT-PCR.The expression of p65 protein was detected by Western blot.Meanwhile,the activity of transcription factor NF-κB was detected with method of luciferase reporter gene.Results：1 μg/L SVC-Ⅲ could promote the p65 expression at both mRNA and protein level,enhanced the activity of transcription factor NF-κB.However,there was no effect at concentration of 10 μg/L and there was an inhabitory effect at concentration of 100 μg/L on NF-κB activity.Conclusion：SVC-Ⅲ could regulate the expression and activity of transcription factor NF-κB in dose-dependent ways in THP1 cells.