期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

中国HIV感染长期不进展者T细胞表面IL-7受体表达研究 被引量：1

Study on the expression of the IL-7 receptor on T lymphocytes in HIV infected Long-Term Non-Progressors
下载PDF
导出
摘要 目的:了解中国HIV感染长期不进展者(Long-Term Non-Progressor,LTNP)T细胞表面IL-7(Interleukin-7,IL-7)受体的表达,分析其与疾病进展的关系。方法:收集LTNP组、HIV组、AIDS组及健康对照组(每组各20例)的抗凝全血,用流式细胞仪检测IL-7受体的表达,并分析IL-7受体表达与血浆中IL-7水平的相关性。结果:LTNP组CD4+T、CD8+T、中枢记忆T细胞(Central memory T cells,Tcm)、效应记忆性T细胞(Effector memory T cells,Tem)表面IL-7受体表达水平明显高于无症状HIV感染组(HIV组)、AIDS病人组(AIDS组)(P<0.05),LTNP组初始T细胞(Na ve T cells,Na ve)、终末分化效应记忆型T细胞(Terminallydifferentiated effector memory T cells,Tem/td)表面IL-7受体表达水平与HIV组、AIDS组间差异无统计学意义。HIV/AIDS患者IL-7受体在CD4+T和CD8+T细胞上表达的百分率与血浆IL-7水平呈明显负相关(P<0.05),与CD4+T细胞数量呈明显的正相关(P<0.05),与病毒载量呈明显负相关(P<0.05)。结论:外周血T细胞表面IL-7受体表达与HIV疾病进展密切相关,LTNP组T细胞保持较高水平的IL-7受体表达,可能是保护因素之一。 Objective：To investigate the expression of Interleukin-7（IL-7） receptor on T lymphocytes of Long-Term Non-Progressors（LTNP）and its association with disease progression.Methods：LTNP,HIV,AIDS patients and healthy controls（20 patients for each group） were enrolled and flow cytometry was used to detect the IL-7 receptor expression on T lymphocytes of whole blood samples taken from the patients and the controls.The association between IL-7 receptor expression on T lymphocytes and IL-7 level in plasma was analyzed.Results：The expression of IL-7 receptor on CD4 and CD8＋T lymphocytes and central memory T cells（Tcm）,effector memory T cells（Tem） of LTNP group was significantly higher than that of AIDS and HIV groups（P〈0.05）;The expression of IL-7 receptor on Nave and Terminally differentiated effector memory T cells（Tem/td） of LTNP group was not statistically different from that of AIDS,HIV groups and healthy controls.The percentage of IL-7 receptor expression on CD4,CD8＋T cells of HIV/AIDS patients negatively correlated with IL-7 level in plasma and HIV virus load and positively correlated with CD4 absolute value（P〈0.05）.Conclusion：The expression of the IL-7 receptor on T lymphocytes from peripheral blood is significantly correlated with HIV disease progression and higher level of IL-7 receptor expression on T cells of LTNPs may play a role in slowing down the disease progression.
作者 李革飞 张子宁 张旻 刘静 施万英 金鑫 姜拥军 王亚男 尚红
机构地区 中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2011年第1期60-63,69,共5页 Chinese Journal of Immunology
基金 国家科技重大专项课题资助项目(2008ZX10001-001) 国家重点基础研究发展计划(973计划)(2006CB504206) 辽宁省高等学校攀登学者(辽教发[2008]90号) 辽宁省重点实验室项目(2008S242)资助
关键词 人类免疫缺陷病毒 IL-7受体 长期不进展 HIV IL-7 receptor Long-Term Non-Progressor（LTNP）
分类号 R512.91 [医药卫生—内科学]
  • 相关文献

参考文献24

  • 1Gorgun G,van der Spek J,Cosenza L et al.Alteredbiological activity associated with C-terminal modifications of IL-7[J].Cytokine,2002;20(1):17-22.
  • 2Llano A,Barretina J,Gutierrez A et al.Interleukin-7 in plasma correlates with CD4 T-cell depletion and may be associated with emergence of syncytium-inducing variants in human immunodeficiency virus type1-positive individuals[J].J Virol,2001;75(21):10319-10325.
  • 3Juffroy O,Bugault F,Lambotte O et al.Dual mechanism for the impairment of IL-7 responses in HIV infection:decreased IL-7 binding and abnormal activation of the JAK/STAT5 pathway[J].J Virol,2010;84(1):96-108.
  • 4Resino S,Correa R,Bellon J M et al.Preserved immune system in long-term asymptomatic vertically HIV-1 infected children[J].Clin Exp Immunol,2003;132:105-112.
  • 5Resino S,Navarro J,Bellon J M et al.Relationship between T-cells subsets and prognostic markers in HIV-1-infected children[J].Med Clin (Barc),2001;117(6):201-206.
  • 6Donna M,Ronald J B,Ryung S K et al.Immunophenotypic markers and antiretroviral therapy (IMART):T cell activation and maturation help predict treatment response[J].JID,2004;189(15):1811-1820.
  • 7Rethi B,Fluur C,Atlas A et al.Loss of IL-7Ra is associated with CD4 T-cell depletion,high interleukin-7 levels and CD28 down-regulation in HIV infected patients[J].AIDS,2005;19:2077-2086.
  • 8Koesters S A,Alimonti J B,Wachihi C et al.IL-7Ra expression on CD4^+ T lymphocytes decreases with HIV disease progression and inversely correlates with immune activation[J].Eur J Immunol,2006;36:336-344.
  • 9MacPherson P A,Fex C,Sanchez-Dardon J et al.Interleukin-7 receptor expression on CD8^+ T cells is reduced in HIV infection and partially restored with effective antiretroviral therapy[J].AIDS,2001;28(5):454-457.
  • 10Kaech S M,Tan J T,Wherry E J et al.Selective expression of the interleukin 7 receptor identifies effector CD8 T cells that give rise to long-lived memory cells[J].Nat Immunol,2003;4:1191-1198.

二级参考文献12

  • 1Schluns KS, Kieper WC, Jameson SC, et al. Interleukin-7 mediates the homeostasis of nave and memory CD8 T cells in vivo. Nat Immunol, 2001, 1: 426-432.
  • 2Napolitano L, Grant RM, Deeks SG, et al. Increased production of IL-7 accompanies HIV-1-mediated T cell depletion: implication for T-cell homeostasis. Nat Med, 2001, 7(1): 73-79.
  • 3Muthukumarl A, Zhou D, Milush J, et al. Role for interleukin-7 in maintenance of T cell homeostasis during SIV infection of a monkey species that does not progress to simian AIDS. 15th AIDS Conference, 2004, ThOrA1443.
  • 4Koot M, Keet IP, Vos AH, et al. Prognostic value of HIV-1 syncytium-inducing phenotype for rate of CD4^+ cell depletion and progression to AIDS. Ann Intern Med, 1993, 118: 681-688.
  • 5Chuitemaker H, Koot M, Kootstra NA, et al. Biological phenotype of human immunodeficiency virus type 1 clones at different stages of HIV-1 infection: progression of disease is associated with a shift from monocytotropic to T-cell tropic virus populations. J Virol, 1992, 66: 1354-1360.
  • 6Komschies KL, Grzegorzewski KJ, Wiltrout RH. Diverse immunological and hematological effects of interleukin7: implication for clinical application. J Leukocyte Biol, 1995, 58: 623-633.
  • 7Kroncke RH, Loppnow HD, Flad M, et al. Human follicular dendritic cells and vascular cells produce interleukin7: a potential role for interleukin-7 in the germinal center reaction. Eur J Immunol, 1996, 26: 2541-2549.
  • 8Biasin1 M, Schenal M, Lisson F, et al. In vitro effects of IL-7 on post-thymic maturation pathways. 15th AIDS Conference, 2004, MoOrA1045.
  • 9Llano A, Barretina J, Gutierrez A, et al. Interleukin-7 in plasm correates with CD4 T-cell depletion and may be associated with emergence of syncytium-inducing variants in human immunodeficiency virus type 1-positive individuals. J Virol, 2001, 75(21): 10319-10325.
  • 10Schmitt N, Chene L, Boutolleau D, et al. Positive regulation of expression and signaling by interleukin-7 in CD4+ mature thymocytes correlates with their capasity to favor human immunodeficiency X4 virus replication. J Virol, 2003, 77(10): 5784-5793.

同被引文献6

引证文献1

二级引证文献1

中国免疫学杂志
2011年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部