新疆阿魏种子无菌苗制备研究

Research on Aseptic Seedling Preparation of Feralu sinkiangensis Seed

为获得供新疆阿魏组织培养无菌苗制备的方法。研究了不同的消毒方法、激素、层积温度和相对湿度等条件下,对新疆阿魏无菌苗制作的影响。结果表明:新疆阿魏种子最佳消毒方法为70%酒精30 s+12%H2O215 min;层积温度4℃,相对湿度50%,新疆阿魏种子萌发最好;新疆阿魏种子经GA3处理可打破休眠,但发芽率低于低温层积处理。

In order to obtain methods of aseptic seedling preparation by tissue culture for Feralu sinkiangensis.Differents plans of sterilization,hormone,temperature of chill and relative humidity were setted to study the effects on germination of Ferula sinkiangensis seed.The results showed that the best sterilization of Ferula sinkiangensis seed was 70%ethanol 30 s＋12%H2O2 15 min.The effect at 4 ℃chilling temperature and in 50% relvtive humidity was better than others treatment,and seed germination rate declined with temperature hoisted.Seed didn＇t germinate when chill temperature preponderate over 20 ℃.Although GA3 could break dormancy of Ferula sinkiangensis seed,germination rate was lower.