DhHP-6对宫内窘迫胎鼠脑保护作用

The protective effect of DhHP-6 on fetal rat brain in intrauterine distress

目的:研究亚血红素6肽(DhHP-6)对宫内缺血缺氧胎鼠脑组织自由基和细胞凋亡的影响,探讨DhHP-6对宫内窘迫胎鼠脑组织的保护作用。方法:Wistar雌性大白鼠,于妊娠第19天通过无损伤动脉钳钳夹双侧子宫、卵巢血管20min建立缺血缺氧宫内窘迫模型,将母鼠随机分为A组(假手术组),B组(缺血缺氧组),DhHP-6治疗组,又分为HI前30 min和HI后立即给药组(即C、D组)。此后实验用母鼠均关腹放回原饲养环境中24h后,立即剖宫取胎,每组随机抽取10只胎鼠断头取脑组织制备标本,行HE染色和TUNEL染色法检查,高倍镜下观察胎鼠脑组织病理改变与海马区神经细胞凋亡情况,剩余胎鼠脑组织冻存,制作匀浆后以比色法测定MDA含量。结果:胎鼠死亡率、MDA含量、海马区细胞凋亡数B组与A组比较,差异具有统计学意义(P<0.01);C组、D组与B组比较,差异有统计学意义(P<0.01、P<0.05);D组MDA含量、细胞凋亡数与C组比较差异具有统计学意义(P<0.01)。结论:DhHP-6有抗氧化和较强的氧自由基清除作用,同时减轻脑组织在宫内已加重的细胞凋亡损伤;缺血缺氧性脑损伤前用药比其后用药效果更佳。

Objective ： To investigate the effects of DhHP - 6 on free radical and apoptosis of fetal rat brain suffered from intrauterine hypoxic -ischemic injury, so as to explore the neuroprotective mechanisms of DhHP -6. Methods： The model of hypoxic -isehemic fetal distress for the wistay female rats was established in the 19 d of pregnancy. The rats were randomly divided into group A （control group） , group B （HI group） and DhHP-6 group （treatment group） ; and divided also into group C （30rain before HI） and group D （immediately after HI） . Experimental rats were then abdominal closure feeding back into the original environment after 24h, 10 fetal rats in each group were randomly selected and taken immediate caesarean births, broken prepared from brain tissue specimens, the pathological changes of brain tissues were observed under light microscope by HE staining and TUNEL assay , the remaining fetal rat brain tissue after cryopreservation, the production was detected after homogenate MDA content. Results ： The differences of fetal rat mortality, MDA content in hippocampus of apoptosis between group B and group A were significant （ P 〈 0. 01 ） , also between group B and group C, group D were significant （P 〈0.01 ,P 〈0. 05）; MDA content and the number of apoptosis in group D was significant difference with in group C （P 〈0. 01） . Conclusion： DhHP - 6 has the effect of against oxidative damage and can reduce the number of apoptosis cells of fetal brain. The effect of using drug before hypoxie -ischemie brain damage is better than later.