摘要
用饱满的微型观赏石榴种子为材料,以MS为基础培养基,添加不同浓度植物生长调节剂(NAA、6-BA)对种子进行无菌萌发试验,并对形成的愈伤组织及试管植株进行快速增殖和生根研究。结果表明:微型观赏石榴种子无菌萌发的适宜配方为MS+6-BA 3.0mg/L+NAA 1.0mg/L+AC 0.3mg/L;最佳增殖配方为MS+6-BA 1.5mg/L+KT 1.5mg/L+NAA 0.5mg/L;最佳生根配方为MS+6-BA 0.5mg/L+IBA 1.5～2.0mg/L+NAA 1.5～2.0mg/L。
Used flush tiny pomegranate seeds as materials,took MS as basic medium,and added with different concentration of plant growth regulators（NAA,6-BA） to test sterile germination,carried out rapid proliferation and rooting study on callus formation.The results showed that the suitable formula for ornamental pomegranate seed germination was MS＋6-BA 3.0 mg/L＋NAA 1.0 mg/L＋AC 0.3 mg/L;the best formula for multiplication was MS＋ 6-BA 1.5 mg/L＋KT 1.5 mg/L＋NAA 0.5 mg/L;the best formula for rooting was MS＋6-BA 0.5 mg/L＋IBA 1.5～2.0 mg/L＋NAA 1.5～2.0 mg/L。
出处
《北方园艺》
CAS
北大核心
2011年第3期153-155,共3页
Northern Horticulture
关键词
微型观赏石榴
组培
种子无菌萌发
增殖
生根
tiny ornamental pomegranate
tissue culture
sterile seed germination
multiplication
rooting
