摘要
以芒草(Miscanthus.sinensis Andress)为试验材料,研究60Co-γ辐照和纤维素酶对其糖化效率的影响。通过对辐照剂量、加酶量、酶解温度、酶解时间和固液比5个因素进行单因素试验,对芒草酶解液中还原糖含量进行检测,最后通过正交试验对酶解条件进行优化。试验结果表明:未经辐照时,当加酶量为240U/g,酶解温度40℃,酶解时间60h,固液比1∶65,芒草的还原糖产量仅为132.59mg/g;而当辐照剂量为800kGy,加酶量150U/g,酶解温度40℃,酶解时间60h,固液比1∶55,还原糖产量可达281.37mg/g。
The effects of ^60Co-γ irradiation and cellulose hydrolysis on the saccharification of Chinese silvergrass were investigated in this paper. The optimal conditions including irradiation dosage, enzyme amount, enzyme hydrolysis temperature, enzyme hydrolysis time and solid/liquid ratio were obtained by orthogonal experiments. The results showed that the reducing sugar production was 132.59mg/g when Chinese silvergrass was treated with 270U/g cellulase at 40℃ for 60h and solid/liquid ratio was 1：65 without irradiation. The reducing sugar production was 281.37mg/g when Chinese silvergrass was treated with 800kGy irradiation and 150U/g cellulase at 40℃ for 60h and solid-liquid ratio was 1：55.
出处
《中国酿造》
CAS
北大核心
2011年第2期29-32,共4页
China Brewing
基金
国家973计划(2009CB226108)
作物种质创新与资源利用国家重点实验室培育基地重点项目(10KFXM02)
湖南省科技计划项目(2010FJ4073)
关键词
芒草
辐照
酶解
还原糖
Chinese silvergrass
irradiation
enzyme hydrolysis
reducing sugar
