太空诱变复合菌剂发酵苹果渣生产菌体蛋白的研究

Production of microbial protein from apple pomace by multi-microbial agent with space mutagenesis

试验利用太空诱变获得的优良菌种--黑曲霉ZM-8和啤酒酵母、热带假丝酵母以及白地霉复配制成复合菌剂,固体发酵苹果渣,得到的菌体蛋白中纤维素含量降低,粗蛋白含量显著提高,营养价值增加。通过正交试验获得了最适的培养基配方和复合菌剂的最佳配方。试验确定的最适培养基配方为白糖4g,硫铵7g,尿素1g,食盐2g,产物中粗蛋白的含量达26.4%,提高了306.3%。复合菌剂的最佳配方为黑曲霉突变株ZM-8(Asp.niger ZM-8)1.5g,酿酒酵母(Saccharomyces cerevisiae)0.5g,热带假丝酵母(Candida tropicalis)1.0g,白地霉菌(Geotrichum candidum)0.5g,产物中粗蛋白的含量达27.9%,提高了337.5%。

The high content of crude protein was produced from the apple pomace fermented by multi-microbial agent including Aspergillus niger ZM-8, Saecharomyces cerevisiae, Candida tropicalis and Geotrichum eandidum, which were treated by space mutagenesis. The optimal formula of complex microbial agent were as followed： A. niger ZM-8 1.Sg, S. cerevisiae 0.Sg, C. tropicalis 1.0g and G. eandidum 0.Sg. The crude protein content of the product reached 27.9%, which was increased by 337.5% compared to that before optimization. The optimal medium was as followed： sugar 4g, ammonium sulphate 7g, urea lg and salt 2g. Under these conditions, the crude protein content of the product was 26.4%, which was increased by 306.3% compared to that before optimization.