海洋放线菌的筛选和抑菌活性的发酵条件优化

Isolation and fermentation optimization of marine actinomycetes with antimicrobial activity

从大连海域筛出33株放线菌,其中4株具有显著地抑菌活性,放线菌D09的抑菌活性最高,并具有广谱抗菌性。以金黄色葡萄球菌为指示菌,对放线菌D09发酵条件和理化性质进行了研究,正交试验表明,其最优发酵条件为:菌龄为48h,接种量10.0%,发酵培养基初始pH值为7,培养条件为25℃,装样量为10%,发酵培养基配方为可溶性淀粉15g/L,KNO3 1g/L,K2HPO4.H2O 0.25g/L,MgSO4.7H2O0.25g/L。在最优条件下,放线菌D09生长最快,抑菌能力最强。

33 marine actinomycetes were isolated from marine mud samples collected from different Dalian sea areas. And 4 of the isolated marine actinomycetes had antimicrobial activities. The strain named D09 had highest antimicrobial activity and a broad antimicrobial spectrum. Using Staphylococcus aureus as a indicator, the fermentation conditions and physieoehemieal properties of D09 were studied. The optimum fermentation conditions for the production of antimicrobial compounds were determined by orthogonal tests as follows： seed age 48h, inoculum 10.0%, initial pH value 7, fermentation temperature 25℃, medium volume 10%, and fermentation medium containing soluble starch 15g/L, KNO3 1g/L, K2HPO4.H2O 0.25g/L, MgSO4-7H20 0.25g/L. Under optimal conditions, both of the growth speed and antimicrobial activity of D09 were highest.