大豆对大豆疫霉菌株Pm14抗性的遗传分析及基因定位

Genetic Analysis and Mapping of Resistance to Phytophthora sojae of Pm14 in Soybean

【目的】研究大豆对大豆疫霉菌株Pm14的遗传模式,并对其抗性基因进行定位。【方法】采用下胚轴创伤接种方法进行抗性鉴定,利用苏88-M21与新沂小黑豆构建的重组自交系群体进行遗传分析,并通过SSR技术结合BSA方法对苏88-M21的抗性基因进行定位。【结果】苏88-M21对大豆疫霉菌株Pm14的抗性由1对主效基因控制,抗病对感病表现为显性。通过分子作图,将抗性基因RpsSu定位于大豆分子遗传图谱O连锁群微卫星标记Satt358和Sat_242之间,与这2个标记的遗传距离分别为3.5 cM和7.4 cM。【结论】苏88-M21对大豆疫霉菌株Pm14的抗性是由1对单显性基因控制的,并将在苏88-M21发现的抗性基因RpsSu定位于O连锁群。

【Objective】The objective of this study was to reveal the inheritance pattern and map resistance gene to Phytophthora sojae（P.sojae） strain Pm14 in soybean.【Method】A population of 176 recombinant inbred lines（RIL） derived from the cross of Su88-M21×Xinyixiaoheidou was evaluated for phytophthora resistance using the hypocotyl inoculation technique.The RIL was used to analyze the inheritance of resistance and map the resistance gene to a linkage group.【Result】The results showed that the resistance of Su88-M21 to strain Pm14 is controlled by a single gene（Rps）,and the resistance is dominant to susceptible.RpsSu（resistance gene in Su88-M21 was temporarily designated） is a novel phytophthora resistance gene which was firstly found on molecular linkage group O,and flanked by Satt358 and Sat_242 with genetic distances 3.5 cM and 7.4 cM.【Conclusion】The resistance to Pm14 is controlled by a single dominant gene（Rps）,and the resistance gene RpsSu in Su88-M21 was firstly mapped on molecular linkage group O.