部分耐冬山茶栽培品种的AFLP分析

Study on the Genetic Diversity of Some Camellia japonica(Naidong) Cultivars Based on AFLP Markers

【目的】从分子水平分析耐冬山茶品种间的遗传关系,探讨其品种分类问题。【方法】利用荧光AFLP技术,采用8对EcoRⅠ/Mse 1引物对耐冬山茶16个品种及2个变异类型、5个山茶南方品种、2个云南山茶品种进行AFLP分析,得到清晰的相关AFLP谱带。【结果】在所测试的30个样品基因组中,8对AFLP引物共获得1 062条清晰的谱带,记录了987条多态性带,多态性带占总带数的92.9%,按照非加权算术平均数聚类法(UPGMA)构建树状分支图。【结论】耐冬山茶与南方山茶的亲缘关系较近;可将耐冬山茶品种分成6个亲缘关系不同的组,表明耐冬山茶有丰富的遗传变异。

【Objective】The genetic diversity of Camellia Japonica（Naidong） cultivar accessions was analyzed at molecular level,and the helpful information for cultivar classification was obtained.【Method】The genetic diversity of 16 cultivars and 2 mutation types of Camellia Japonica（Naidong） with 5 cultivars of C.japonica from south of China,2 cultivars of C.reticulata was analyzed by the fluorescent-AFLP marker techniques with 8 pairs of EcoRⅠ/Mse 1 primer combinations.【Result】In the genomes of the 30 tested samples,a total of 1 062 spectral bands amplified from the 8 pairs of primer combinations were selected from AFLP analysis and 987 bands appeared to be polymorphic（92.9%）.The data were used to calculate the Nei＇s genetic distance and to construct a dendrogram based on UPGMA cluster analysis.【Conclusion】It showed that the cultivars of Camellia japonica（Naidong） have a remote genetic relationship compared with the cultivars of Camellia from south of China,and cultivars of Camellia japonica（Naidong） can be divided into 6 groups,which indicated that Camellia japonica（Naidong） have rich genetic variability.