摘要
目的建立HPLC法测定盐酸林可霉素注射液中苯甲醇的含量。方法采用DiamonsilTM（钻石）C18色谱柱;流动相：0.05mol.L-1硼砂溶液（用85%磷酸溶液调节pH值至5.0）-甲醇-乙腈（67∶33∶2）,检测波长：214nm;进样量：10μL。结果苯甲醇在0.01903~0.32572mg.mL-1范围内线性关系良好（r=0.9999）,平均加样回收率为100.6%。结论该方法操作简便、准确、重复性好,可用于测定盐酸林可霉素注射液中苯甲醇的含量。
Objective To establish the determination method of benzyl alcohol in lincomycin hydrochloride injection by HPLC.Methods DiamonsilTM C18 column was used.0.05mol·L-1 borax solution（adjust to pH 5.0 with 85% phosphoric acid）-methanol-acetonitrile（67∶33∶2） was mixed well as mobile phase.Detection wavelength was 214nm.The injection volume was 10μL.Results The linear range of benzyl alcohol was within 0.01903～0.32572mg·mL-1（r=0.9999）.The average recovery was 100.6%.Conclusion The method is simple,rapid and accurate with good reproducibility.It is suitable for the quality control of benzyl alcohol in Lincomycin Hydrochloride Injection.
出处
《中国药事》
CAS
2011年第1期73-75,共3页
Chinese Pharmaceutical Affairs
