摘要
采用95%乙醇加热回流提取唐古特白刺(Nitraria tangutorum Bohr.)果实成分,依次用石油醚、氯仿、乙酸乙酯和正丁醇进行萃取。用DPPH自由基法和ABTS+·法进行抗氧化活性实验,并选取抗坏血酸(VC)和生育酚(VE)作为对照。结果显示乙酸乙酯提取物(EAF)具有较高的抗氧化性,进一步对其进行硅胶柱层析分离,得到6个组分(EAF-Ⅰ～EAF-Ⅵ),其中EAF-Ⅰ和EAF-Ⅳ的抗氧化性均高于VC和VE。白刺果实提取物具有良好的抗氧化性,有进一步开发为天然抗氧化剂的应用前景。
Nitraria tangutorum Bohr. fruits were extracted using 95% ethanol, and then the extract was partitioned with petroleum ether, chloroform, ethyl acetate and n-butanol successively. The antioxidant activities of different fractions were evaluated by scavenging capability of ABTS~ and DPPH free radicals, and compared with the antioxidant activities of ascorbic acid （vitamin C） and tocopherol （vitamin E）. Results indicated that ethyl acetate fraction （EAF） exhibited the highest antioxidant activity. EAF was further fractionated into 6 fractions by silica gel column chromatography. The antioxidant activities of EAF- I and EAF-IV were higher than those of vitamin C and vitamin E. Therefore, N. tangutorum fruit extracts have excellent antioxidant activities, and have promising application prospects as a natural antioxidant.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第3期95-97,共3页
Food Science
基金
国家自然科学基金项目(30660015)
高等学校博士学科点专项科研基金项目(20091501110002)
内蒙古自治区科技创新引导奖励资金(2010年度)资助项目
关键词
唐古特白刺果实
DPPH自由基
ABTS+·
抗氧化性
分离提取
Nitraria tangutorum Bohr. fruit
DPPH free radical
ABTS^＋ free radical
antioxidant activity
separation and purification
