长双歧杆菌BBMN68对便秘模型小鼠的通便作用

Effect of Biofidobactria Strain on Defecating Function in Constipation Mice

目的:研究长双歧杆菌活菌BBMN68活菌液对便秘模型小鼠的润肠通便作用。方法:将Balb/c雄性小鼠随机分为空白组、模型组、阳性对照组和3个剂量的给药组。给药组分别经口给予低、中、高剂量(1×107、1×108、1×109CFU/mL)的长双歧杆菌BBMB68活菌液0.1mL/(kg·d)(以体质量计);空白组和模型组给予0.1mL/(kg·d)的质量分数10%的脱脂奶溶液;阳性对照组给予1×108CFU/mL动物双歧杆菌菌悬液0.1mL/(kg·d)。用复方地芬诺酯制造小鼠便秘模型,建模后各组分别给药,观察各组小鼠的首次排黑便时间、5h内排便粒数和质量,测定粪便含水量和小肠墨汁推进率。结果:长双歧杆菌BBMN68给药7d,可明显改善便秘小鼠首次排黑便时间、增加排黑便粒数和质量;长双歧杆菌BBMN68给药15d可显著提高便秘小鼠小肠墨汁推进率。表明长双歧杆菌BBMN68具有润肠通便功能。

The objective of this study was to evaluate the defecating function of Biofidobacterium animal BBMN68 on constipated mice. Mate Balb/c mice were randomly divided into control group, model control group, positive control group and 3 dosages groups. Mice of 3 dosages groups were orally administered with different concentrations of live B. BBMN68 （1×10^7, 1×10^8, 1 × l0^9 CFU/mL） at 0.1 mL/（kg＊d） dose once a day, and mice of control group as well as model control group were administered with 10% skim milk liquid 0. lmL/（kg＊d）, while the mice of positive control group with 1×10^8 CFU/mL B. animal at 0.1 mL/（kg＊d）. Mice constipation model was established by using compound diphenoxylate. Each group was respectively treated with corresponding drugs, and then the time of defecation of the first black feces, the character of feces within 5 hours, and the promoting rate of ink were measured. Results： the mice administrated with B. BBMN68 for 7 days had the shorter time of defecation of the first black feces, and the number and weight of mice feces within 5 hours were increased; when the mice were administered with BBMN68 strain for 15 days, the ink propelling rates were increased. Conclusion： B. animal strain BBMN68 strain has the potential defecation function in constipation mice.