摄食鱿鱼对乳清酸诱导大鼠脂肪肝的影响

Effects of Dietary Squid on Lipid Metabolism in Rats with Orotic Acid-induced Fatty Liver

目的:研究摄食鱿鱼对脂肪肝大鼠脂肪代谢的影响。方法:24只大鼠分成3组,对照组喂食AIN76标准饲料,模型组饲料中添加1%乳清酸,鱿鱼组添加1%乳清酸和10%鱿鱼。饲喂10d后,分别测定大鼠血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)含量,以及肝脏TC、TG、磷脂含量。同时测定肝脏中脂肪酸合成酶(FAS)、苹果酸酶(ME)、葡萄糖6磷酸脱氢酶(G6PDH)、肉碱棕榈酰转移酶(CPT-1)和过氧化物酶体β-氧化酶系活性。结果:乳清酸可显著升高大鼠肝脏以及血清TC、TG含量,升高FAS、ME和G6PDH活性,并降低CPT-1的活性。添加鱿鱼后,显著降低了乳清酸诱导的脂肪肝模型大鼠的肝脏以及血清TC、TG水平,降低了FAS、ME、G6PDH活性并升高了过氧化物酶体β-氧化酶系活性。摄食乳清酸降低了大鼠总脂和中性固醇的排泄,摄食鱿鱼后对大鼠总脂排泄无显著影响,但降低了中性固醇的排泄。结论:摄食鱿鱼可以通过降低乳清酸诱导的脂肪肝模型大鼠的脂肪酸合成酶活性和升高过氧化物酶体β-氧化酶系活性来抑制脂肪肝大鼠肝脏脂质蓄积。

Objective： To investigate the effect of dietary squid on lipids metabolism in fatty liver in rats. Methods： Twenty four male SD rats were randomly divided into 3 groups and control group. The control was fed AIN76 diet and model group AIN76 diet plus 1% orotic acid, while squid group AIN76 diet plus 1% orotic acid and 10% squid. After 10 days, serum triglyceride （TG）, total cholesterol （TC）, and high density lipoprotein cholesterol （HDL-C） levels were determined. Hepatic lipid concen- trations （TG, TC, PL） and the activities of malic enzyme （ME）, glucose-6-phosphate dehydrogenase （G6PDH）, fatty acid synthase （FAS）, camitin palmitoyl transferase （CPT）, peroxisomal β-oxidation enzymes were also examined. Results： After fed orotic acid, liver TC and TG levels were significantly increased, so did the activity of FAS, ME and G6PDH, but the activity of CPT drop. Taking squid could reduce the liver TC and TG and the activity of FAS, ME, G6PDH but increased the activity of peroxisomal β-oxidation enzymes in orotic acid-induced fatty liver in rats. Conclusion： This study indicated that the dietary squid could inhibit liver lipids cumulating in orotic acid-induced fatty liver in rats by reducing the activity of FAS, ME and G6PDH and increasing the activity of peroxiso mal β-oxidation enzymes.