黄檗果皮提取物化感作用的研究

Study on allelopathy effect of pericarp extract of Phellodendron amurense

目的:通过对黄檗果皮化感作用的研究,探讨其在黄檗野生种群自我更新障碍中的作用,为黄檗野生资源的保护寻求方法和途径。方法:用4℃蒸馏水浸泡(水浸液)及蒸馏水回流提取(水提液)的黄檗果皮提取液培养白菜、小麦种子,研究其对受体种子萌发及幼苗生长的作用。结果:20 g.L-1水浸液及水提液对白菜、小麦种子的萌发有显著的抑制作用,100g.L-1时完全抑制小麦的萌发;20 g.L-1水浸液显著抑制白菜、小麦的生长,完全抑制白菜根的生长,100 g.L-1水浸液完全抑制白菜、小麦的生长;相比于水浸液,水提液对种子萌发及幼苗生长的作用强度都有所减弱。结论:黄檗果皮的化感作用是多物质作用的结果,部分化感物质对热敏感;黄檗果皮的化感作用总体上表现为对种子萌发及幼苗生长的抑制,推测黄檗果皮的物理及化学特性是致使其种群自我更新困难的因素之一。

Through the study of allelopathy of the pericarp of Phellodendron amurense, the role of serf-regeneration barriers was investigated in order to find ways and means for the protection of wild populations of P. amurense. Solution preparation： soaked peri- carp of P. amurense in distilled water at 4 ~C to get solution A ,and reflux extraction of pericarp with distilled water at 100 ~C to get so- lution B. Both of the solution A and solution B were used in the experiment of seed germination and seedling growth with the seeds of cabbage and wheat. The results showed that 20 g ~ L-1 concentration of solution A and solution B inhibited significantly seed germina- tion of cabbage and wheat, while 100 g ~ L-i concentration of solution A even completely inhibited the seed germination of wheat. 20 g ~ L i concentration of solution A significantly inhibited the cabbage and wheat seedling growth, completely inhibited the root growth of cabbage, while 100 g ·L-1 concentrations of solution A completely inhibited seedling growth of cabbage and wheat. Comparing to solution A, the intensity of solution B are diminished on seed germination and seedling growth. It is concluded that the allelopathy of pericarp of P. amurense is multi-material role in the results, some of allelochemicals are easily degradable when exposed to heat. Overall, the allelopathy of pericarp of P. amurense can affect the seed germination and seedling growth. It is supposed that allelochemicals existed in the pericarp of P. amurense is one of the reason leading to difficulties in self-regeneration of its population.