头花蓼不同地理居群的叶绿体psbA-trnH和trnL-trnF基因序列与聚类分析

Analysis on chloroplast DNA sequences of Polygonum capitatum of different geographical population

目的:探讨民族药头花蓼居群间的DNA序列变异与地理分布的关系,为头花蓼优良种质资源的筛选评价提供分子依据。方法:采用PCR产物直接测序,对头花蓼11个居群11个个体样品的叶绿体psbA-trnH,trnL-trnF基因进行测序分析研究。结果:头花蓼11个居群的叶绿体psbA-trnH长402 bp,存在6个变异位点。trnL-trnF长875 bp,存在5个变异位点。用UPGMA法构建的聚类图表明,头花蓼在贵州至云南的类群存在较大程度变异。结论:贵州西部与云南东部地区的头花蓼有利于优良种质资源的筛选。

Objective： To investigate the relationship between the variation of chloroplast DNA gene sequences and the geo- graphical origins of Polygonum capitatum in order to provide the molecular evidence for its excellent germplasm resources. Method ： PCR direct sequencing was applied to detect the chloroplast psbA-trnH, trnL-trnF gene sequence of 11 samples collected from 11 popu- lations of P. capitatum. Result： The psbA-trnH gene sequence of P. capitatum from different populations was 402 bp in length, there were 6 variable sites. TrnL-F gene sequence was 875 bp, there were 5 variable sites. The clusters diagram by UPGMA method showed that P. capitatum groups in Yunuan and Guizhou existed a considerable variation. Conclusion： P. capitatum which is located in the east of Yunnan and the west of Guizhou is helpful of screening the germplasm resources.