菝葜药理活性部位的纯化

Purification of Pharmacological Active Parts in Smilax china L.

目的确定大孔吸附树脂纯化菝葜药理活性部位并保证其化学成分整体性的最佳工艺。方法以甾体总皂苷的含量为指标,选用D101大孔吸附树脂纯化,采用三级管阵列检测器-高效液相色谱法建立各纯化物色谱图。结果最佳纯化工艺为:先分别用纯化水和3倍柱体积的30%乙醇洗脱去杂质,再收集4倍柱体积的70%乙醇洗脱液得皂苷,最后用95%乙醇清洗树脂。结论该纯化工艺所得纯化物的纯度高,化学成分整体性好。

Objective To find out the best process for purification of pharmacological active parts from Smilax china L.with macroporous adsorptive resins and biochemical integrity. Methods D101 macroporous adsorptive resin was used to purify compounds and the corresponding chromatogram maps were set up with DAD-HPLC according to the content of total steroidal saponins. Results The best procedure was that eluted the compounds with distilled water and 3BV 30% ethanol to eliminate the impurities,collected the 4BV 70% ethanol eluent to gain the steroid saponins and wash the resin with 95% ethanol at last. Conclusion The purified substances obtained from this process are with high purity and good biochemical integrity.