不同型号大孔吸附树脂对何首乌二苯乙烯苷的吸附性考察

The Study of Adsorptive Properties of TSG from Polygonum Multiflorum Thunb.with Different Types of Macroporous Absorption Resin

目的优选适合纯化何首乌水提液的大孔吸附树脂。方法以二苯乙烯苷保留率为评价指标,采用高效液相色谱法测定何首乌提取液和洗脱液中二苯乙烯苷的含量,通过吸附率、洗脱率和纯化效率优选出适合分离纯化何首乌水提液二苯乙烯苷的最佳树脂类型,并考察该树脂的吸附平衡时间。结果不同型号树脂对何首乌水提液中二苯乙烯苷的吸附效果有差异,D101型树脂对二苯乙烯苷的吸附性能较好,且易洗脱,纯化效率最高。二苯乙烯苷在D101树脂上的吸附平衡时间为7 h。结论 D101型树脂适合分离纯化何首乌水提液。

Objective To optimize the macroporous adsorption resin for purification of water extract of Polygonum multiflorum Thunb. Methods The retention rate of 2,3,5,4＇-tetrahedroxystilbene-2-O-β-D-glucoside was used as the evaluation indicator,content of 2,3,5,4＇-tetrahedroxystilbene-2-O-β-D-glucoside from water extacts and eluents of Polygonum multiflorum Thunb.were determined by HPLC.Through the adsorption rate,elution rate and absorption efficiency to optimize the macroporous adsorption resin for purification of water extracts from Polygonum multiflorum Thunb.and study the adsorption equilibrium time of D101 macroporous absorption resin. Results Different types of macroporous resin for TSG of water extract from Polygonum multiflorum Thunb.were different.The D101 macroporous adsorption resin had the best separation performance and was the most suitable resin for purification of water extract of Polygonum multiflorum Thunb.The adsorption equilibrium time of TSG on D101 was 7 h. Conclusion The D101 macroporous adsorption resin fits for isolation and purification of water extract from Polygonum multiflorum Thunb.