肺部感染患者军团菌属感染的调查

Investigation of patients with pulmonary infection caused by legionella

目的调查肺部感染患者嗜肺军团菌血清Ⅰ型(LENⅠ)尿抗原及军团菌属16S rRNA基因的阳性率。方法采用聚合酶链反应(PCR)扩增肺部感染患者尿液中军团菌属特异性16S rRNA基因,酶免疫法(EIA)检测其尿液中LENⅠ抗原。结果对213例患者进行LENⅠ尿抗原检测,其中10例阳性,阳性率为4.7%,对其进行军团菌属16S rRNA基因PCR扩增,同样有10例阳性,阳性率为4.7%,2种检测方法同时阳性的有3例;以LENⅠ特异性尿抗原或尿液中军团菌属特异性基因扩增结果任一阳性作为军团菌属感染的判定标准,军团菌属感染阳性率为8.0%。结论肺部感染患者中存在一定程度的军团菌属感染,可采用尿抗原及PCR技术进行检测。

OBJECTIVE To investigate the serotype I （LEN I ） of legionella from the patients with pulmonary infection, urinary antigen, gene 16S rRNA and positive rate. METHODS Legionella-specific 16S rRNA gene in the urine of the patients with pulmonary infection was amplified by polymerase chain reaction （PCR）. The LEN antigen I in urine was detected by EIA. RESULTS A total of 10 cases were positive among 213 cases who were conducted the detection of LEN I antigen in urine with the positive rate of 4.7%. 16S rRNA of ten positive cases were amplyied （4. 7%）. There were 3 cases positive at the same time detected by both of the methods. The positive rate of legionella infection was 8. 0% judged by any positive result of legionella-specific antigen or the amplified legionella-specific gene in urine. CONCLUSION The patients with pulmonary infection have legionella infection to a certain degree, which can be detected by adopting urinary antigen and PCR.