流动注射时间扫描荧光分析法测定盐酸二氧丙嗪

A Time Scanning Fluorophotometric Method for the Determination of Dioxopromethaine Hydrochloride by Flow Injection Analysis

研究了硫酸颜色反应用于盐酸二氧丙嗪荧光测定的方法。盐酸二氧丙嗪与浓硫酸反应后,诱发产生强荧光。结合流动注射进样技术,借助时间扫描荧光方式,提出了流动注射时间扫描荧光分析法测定盐酸二氧丙嗪的新方法。体系最大激发和发射波长分别为274.0 nm和376.0 nm。盐酸二氧丙嗪浓度在2.8×10-7～4.0×10-5g/mL范围内与荧光强度呈良好的线性关系,相关系数为0.9998,方法检出限为1.1×10-8g/mL,相对标准偏差为0.5%(n=11,c=1.2×10-6g/mL),进样频率为100次/小时。方法用于药物制剂及尿样中盐酸二氧丙嗪的直接测定,结果满意。

The fluorescent characteristic of the colour reaction between dioxopromethaine hydrochloride and sulfuric acid has been studied.The experiment results indicated that dioxopromethaine hydrochloride was a weak fluorescent substance and could react with sulfuric acid to form an intense fluorescent substance.Combined with flow injection analysis and time scanning technology,a new flow-injection fluorescence method for the determination of dioxopromethaine hydrochloride was established.The experimental conditions that influence the fluorescence intensity were investigated and optimized.The excitation and emission wavelengths were 274.0 and 376.0 nm,respectively.The linear range was 2.8×10-7～4.0×10-5 g/mL with the detection limit of 1.1×10-8 g/mL.The relative standard derivation was 0.5%（n=11,c=1.2×10-6 g/mL）,the frequency of sample introduction was 100 samples/h.The method has been used for the determination of dioxopromethaine hydrochloride in tablets and human urines with satisfactory results.