阿卡波糖片的亲水色谱法测定

Determination of Acarbose Tablets by Hydrophilic-interaction Chromatography

建立了亲水色谱法测定阿卡波糖片的含量及其有关物质。采用Phenomenex氨基柱,以6 mmol/L磷酸二氢钾缓冲液(pH 6.8)-乙腈(28:72)为流动相,检测波长210 nm。阿卡波糖在4～20 mg/ml浓度范围内线性关系良好。平均回收率为99.2%,RSD为0.7%。

A hydrophilic-interaction chromatography method was established for the determination of acarbose tablets and its related substances.A Phenomenex NH_2 column was used with the mobile phase of 6 mmol/L potassium dihydrogen phosphate buffer（pH 6.8） -acetonitrile（28：72）,at the detection wavelength of 210 nm.The calibration curve was linear in the concentration range of 4-20 mg/ml.The average recovery was 99.2%with RSD of 0.7%.