接触性泻剂损伤小肠传输功能的实验研究

An experiment study of the small intestinal transit injuried by contact laxatives

目的 探讨接触性泻剂对小肠传输功能的影响及其病理学机制．方法 采用给大鼠饲料里添加接触性泻剂的方法使大鼠发生腹泻，４５ ｄ 后停止给药，５２ ｄ 后测量大鼠小肠传输功能的改变；并应用免疫组化的方法测定小肠肌间神经丛内血管活性肠肽（ＶＩＰ） 、Ｐ物质和Ｓ－ １００ 蛋白的含量变化．结果 长期大剂量服用接触性泻剂可明显损伤小肠的传输功能，使小肠传输减慢；小肠肌间神经丛内ＳＰ 和Ｓ－ １００ 蛋白的含量增加，而ＶＩＰ无明显变化．结论 长期服用接触性泻剂可明显损害小肠的传输功能。

AIM To investigate the effect of contact laxatives on small intestinal transit and its pathologic mechanism. METHODS Experimented Wistar rats were fed with contact laxatives, rhubarb and phenophthalein, to make the rats have diarrhoea. The laxatives were stopped after 45 days and the rats small intestinal transit change was meassured after 52 days. By using immunohist_ ochemistry, the quantitative changes of vasoactive intestinal peptide (VIP), substance P (SP) and S-100 protein in the small intestinal myenteric plexus were studied. RESULTS The small intestinal transit was markedly injuried, manifesting the slow transit, by the contact laxatives abused for a long time. The content of SP and S-100 protein were increased in the myenteric plexus and the VIP was remained unchanged. CONCLUSION The small intestinal transit may be injuried by the contact laxatives abused, its mechanism was probably as a result of the small intestinal myenteric plexus damaged.