拆方扶正化瘀方对肝细胞及肝星状细胞功能的影响

Effects of coordination of FZHY decoction on functions of hepatocytes and hepatic satellate cells

目的 探讨扶正化瘀方防治慢性肝病的作用机制及药效学配伍意义．方法 将扶正化瘀方分为扶正组、化瘀组、虫草组、丹参组及丹参加虫草组，灌胃给药分离药物血清，温育传一代大鼠肝星状细胞和原代肝细胞． 半定量ＲＴＰＣＲ 法检测Ⅰ型胶原ｍ ＲＮＡ表达，ＥＬＩＳＡ 法检测Ⅰ型胶原和清蛋白分泌，３ ＨＴｄＲ 和３ Ｈ脯氨酸掺入分别观察细胞增殖和活力．结果 各组药物均可抑制星状细胞的增殖，其中化瘀药效果最明显；各组药物均可抑制星状细胞Ⅰ型胶原ｍ ＲＮＡ 和蛋白的生成，以扶正组效果最佳；全方明显促进肝细胞和肝星状细胞蛋白质生成，而各拆方组均无此作用．结论 扶正与化瘀两法配伍组成扶正化瘀方在防治慢性肝病方面具有整体优势．

AIM To investigate the pharmacological mechanism of the coordination of herbs in FZHY decoction concerned in chronic hepatic diseases. METHODS FZHY decoction was divided into five subgroups: Fuzheng, Huayu, Cordyceps, Salvia Mitiorihizae and Cordyceps plus Salvia Mitiorihizae groups. The normal rats were fed on FZHY decoction and subgroups, then the drug sera were collected from the rats, and incubated with subculture of hepatic satellate cells (HSC) and primary cultured hepatocyte. A semi quantitative RT PCR method was used to evaluate type Ⅰ collagen mRNA expression, ELISA method was used to examine type Ⅰ collagen and albumin secretion, HSC proliferation and activation were observed by 3H TdR and 3H proline incorporation respectively. RESULTS Drug sera of all groups inhibited HSC proliferation, Huayu group had the best effect. All groups decreased protein secretion and its mRNA expression of type Ⅰ collagen in HSC, Fuzheng group had the best effects. FZHY decoction enhanced both hepatocyte and HSC protein synthesis, but the rest groups had no this action. CONCLUSION Fuzheng and Huayu herbs have the effects on chronic hepatic diseases respectively, but the combination of Fuzheng and Huayu herbs has comprehensive advantages over the diseases.