高氟对人甲状腺细胞功能的影响

Effect of fluoride on thyroid function in primary thyrocytes

目的探讨高氟对人甲状腺细胞功能的影响。方法用人甲状腺细胞,采用原代培养方法,按氟(NaF)剂量不同分为:0(对照组)、40、80、160 mg/L组。染氟48 h后,采用噻唑蓝(MTT)法测定细胞存活率,化学发光法测定抗甲状腺过氧化物酶抗体(TPOA)水平和荧光免疫分析法测定血清总甲状腺激素(T_4)水平。结果甲状腺细胞染氟培养48 h后,细胞存活率组间比较差异有统计学意义(P<0.05);40 mg/L组细胞存活率与对照组(100)%比较,差异无统计学意义(P>0.05);80、160 mg/L组细胞存活率分别与对照组比较,差异有统计学意义(P<0.05)。甲状腺细胞T_4水平随着染氟剂量的升高而明显降低,组间比较差异有统计学意义(P<0.05)。甲状腺细胞TPOA表达水平也随着染氟剂量的升高而明显增加,组间比较差异有统计学意义(P<0.05)。结论高氟可损伤甲状腺细胞,降低细胞存活率,提高TTPOA表达,降低T_4水平。

Objective To study the effect of fluoride on thyroid function in primary thyrocytes. Methods A sodium fluoride injured model was developed with purified cultured porcine thyrocytes , and the experiment was divided into 0 （control group） ,40,80 and 160 mg/L groups according to the final concentration of NaF. Forty - eight hours after fluoride, the cell survival ratio was measured by methyl thiazolyl tetrazolium（MIT） method and the thyroid peroxidase（TPO） antibody was assayed by chemiluminescence method. Radio - immunoassay was used to detect the thyroxine level （ T4 ）. Results After forty - eight hours of injury of thyroid cells with NaF, there was statistically significant difference in the cell survival ratio between these groups （ P 〈 0.05 ）. The cell survival ratio in the 40mg/L group was not statistically different from that of control group（ P 〉 0.05 ）, but there was statistically significant difference （P 〈 0.05 ）between the 80mg/L and 160mg/L groups. With the increasing dose of fluoride, the thyroxine level of T4 became remark- ably lower and was of statistic significance in group comparison （ P 〈 0.05 ）. The TPO antibody was elevated with the increase of the dose of fluoride（ P 〈 0.05 ）. Conclusion High fluoride of T4 affects the thyroid function by reducing the thyroxine level. The mechanism may lie in inhibiting the cell survival rate and increasing TPO antibody by inducing apoptosis.