高效毛细管电泳法用于麻保沙星的测定

Determination of marbofloxacin by high performance capillary zone electrophoresis

建立了一种高效毛细管电泳法测定麻保沙星原料药含量的方法。采用未涂层石英毛细管柱,以15 mmol/L硼酸钠缓冲液（pH值为9.2）为电泳介质,分离电压为20 kV,采用高度差进样10s,检测波长为295 nm,麻保沙星在10~100μg/mL范围内线性良好,平均回收率为100.2%,RSD值为0.97%。

A high performance capillary zone electrophoresis method was established for the determination of marbofloxacin drug substance.An untreated fused-silica capillary was used with 15 mmol/L sodium borate buffer（pH 9.2） as running buffer at the detection wavelength of 295 nm,separation voltage of 20 kV and the injection volume of sample for 10 s.The calibration curve for marbofloxacin is linear in the concentration range of 10~100 μg/mL.The average recovery is 100.2% with RSD of 0.97%.