电针水沟对脑出血大鼠大脑皮质NPY调节作用的动态观察

Dynamic Observation of the NPY Regulation Effect of Electroacupuncture Shuigou (DU26) in Rats with Cerebral Hemorrhage

目的观察神经肽Y(NPY)在脑出血大鼠大脑皮层神经细胞和内皮细胞中的动态表达,探讨电针水沟穴对脑出血大鼠脑血管神经调节物质NPY的作用及可能机制。方法将健康青年wistar大鼠随机分为正常组、假手术组、模型3、24、72h组、电针3、24、72h组,每组各10只。采用胶原酶Ⅶ诱发的大鼠尾壳核出血模型;电针组取水沟穴治疗,用连续波,频率120次/min,强度1mA,留针30min。造模麻醉醒后立即针刺1次,每隔24h再针刺1次。根据Bederson神经体征评分法进行综合等级评分,并采用免疫组化方法检测各组大鼠皮层组织NPY的动态表达。结果 (1)造模麻醉醒后电针治疗组的神经缺损体征综合评分略高于模型组。3d后,电针治疗组神经缺损体征综合评分与模型组及电针治疗前比较均有明显差异。(2)正常组与假手术组大鼠大脑皮层神经元和内皮细胞有少量的NPY阳性表达;脑出血后3h大鼠大脑皮层NPY的阳性表达开始增高,24h呈最高峰,胞浆和突起浓染呈棕褐色,血管内皮细胞细胞质中有棕色颗粒,72h时有所下降,与正常组比较均有显著差异。电针组大鼠大脑皮层神经元和内皮细胞的NPY阳性表达,也于3h开始表达,于24h表达较高,72h表达减弱,与模型组比较均有显著差异。结论电针水沟穴能有效抑制脑出血后3、24、72h时大脑皮质神经元和血管内皮细胞NPY的表达增强,改善脑出血大鼠的行为体征。

Objective：To investigate the dynamic expression of neuropeptide Y（NPY） in brain cortex and endotheliam of intercerebral hemorrhage rats and explore the effects and the possible mechanism of electroacupuncture Shuigou（DU26） in regulating NPY.Methods：80 wistar rats were divided into 4 groups randomly including the normal group,the sham-operation group,the model group and the electroacupuncture group.The latter two groups were respectively divided into 3 groups by different hours at 3h,24h,and 72h.Rat models of intercerebral hemorrhage were induced by injecting 0.8μL collagenase Ⅶ,which is behind front-fontane 1.0mm,right 4.0mm,deep 5.0mm.And the electroacupuncture group rats were stimulated on Shuigou（DU26） by G6805-II cural apparatus,choosing continune wave with frequency 120times/min,intension 1mA,remaining 30min.This electroacupuncture was treated after anesthesia recovery.Meanwhile,this acupuncture was treated every 24h.The synthesis grade score was used Bederson neurosigns score method.The dynamic expression of NPY in the brain cortex was detected by the immunohistochemistry method.Results：（1） After anesthesia recovery,the neurological defects signs comprehensive score of the electroacupuncture group was higher than that of the model group.After 3 days,there was a statistical difference between the model group and the electroacupuncture group in above scroe.（2）There were a few of NPY positive expression of the brain cortex and endotheliam of intercerebral hemorrhage in the normal group and the sham-operation group.At 3h after intercerebral hemorrhage,the NPY positive expression was increased.At 24h,the expression was at top.There were concentrated dyeing in the cytoplasm and the processes.Meanwhile,the brown particle was in the cytoplasm of the endotheliam.At 72h,these conditions were decreased.There was a statistical difference between the electroacupuncture group and the control group.The electroacupuncture group,the NPY positive expression was appeared at the 3h,gone to top at the 24h and decreased at the 72h.Compared with the model group,there was a statistical difference between 2 groups.Conclusion：Electroacupuncture on Shuigou（DU26） can resist the NPY expression increase in the brain cortex and endotheliam at 3h,24h and 72h after intercerebral hemorrhage.It can improve the behavior signs of thintercerebral hemorrhage rats.