针刺对大鼠脊髓损伤后内源性神经干细胞的影响

Effect of Acupuncture on the Endogenous Neural Stem Cells of Rats with Spinal Cord Injury

目的探讨针刺对大鼠脊髓损伤后内源性神经干细胞(ENSCs)的诱导分化。方法 25只成年SD雄性大鼠,随机分为假手术组、模型组和针刺治疗组。假手术组不损伤脊髓,模型组和针刺治疗组用改良Allen’s重物坠击法制备脊髓损伤模型。模型组不予任何处理;针刺治疗组予以针刺治疗,每日1次,7d为1疗程,共2个疗程。于2周后取材,对距离损伤中心5mm的脊髓行免疫组化染色,检测Nestin、Brdu、NSE、GFAP的表达。结果 Nestin和Brdu的表达针刺治疗组较模型组多,假手术组最少;NSE的表达针刺治疗组较模型组多,但少于假手术组;GFAP的表达模型组高于针刺治疗组,假手术组最低。结论针刺对大鼠脊髓损伤后内源性神经干细胞有诱导分化作用。

Objective：To investigate the differentiation effect of acupuncture on endogenous neural stem cells（ENSCs） after spinal cord injury in rats.Methods：25 SD rats were randomly divided into 3 groups such as the sham-operation group,the model group and the acupuncture group.The Allen＇s method was used to be the spinal cord injury model.After 2 treatment courses,the spinal cords were stained by the immunohistochemical method.And then,expressions of Nestin,Brdu,NSE and GFAP were tested.Results：The Nestin and the Brdu expression of the acupuncture group were more than those of the model group.There were the least expressions in the sham-operation group.The NSE expression in the acupuncture group was more than it in the model group and fewer than it in the sham-operation group.The GFAP expression in the model group was higher than it in the acupuncture group.This least expression was in the sham-operation group.Conclusion：Acupuncture can activate endogenous neural stem cells after spinal cord injury in rats.